Journal: Cell reports
Article Title: SOD2 is a regulator of proteasomal degradation promoting an adaptive cellular starvation response
doi: 10.1016/j.celrep.2025.115434
Figure Lengend Snippet: (A–F, left) Jurkat, MOLT4, OCI-AML2, NALM-16, HCT-15, and SW480 cells were transduced with the indicated shRNAs, and the knockdown efficiency of SOD2 was assessed by RT-qPCR analysis in biological duplicates. (A–F, right) Jurkat, MOLT4, OCI-AML2, NALM-16 HCT-15, and SW480 cells were treated with the indicated doses of asparaginase in biological triplicates. After 8 days of treatment, relative viability was assessed. Cell counts were normalized to shLuc-transduced, vehicle-treated cells. (G–J) Jurkat cells were transduced with the indicated shRNAs and treated with the indicated doses of vincristine, dexamethasone, doxorubicin, and 6-mercaptopurine in biological duplicates. After 8 days of treatment, relative viability was assessed. Cell counts were normalized as in (A–F). All error bars represent SEM. *** p ≤ 0.001, ** p ≤ 0.01, * p < 0.05 by two-sided Student’s t test with Welch adjustment (A–F, left). See also and .
Article Snippet: MOLT4 cells , DSMZ , Cat#ACC-362.
Techniques: Transduction, Knockdown, Quantitative RT-PCR